Difference between revisions of "PWH844"
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*'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | *'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | ||
*'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | *'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | ||
| − | *<font color="#99E500">''' | + | *<font color="#99E500">'''JK37'''</font>: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘ |
<pubmed> 9023219 </pubmed> | <pubmed> 9023219 </pubmed> | ||
== Return to [[Plasmids]] == | == Return to [[Plasmids]] == | ||
Latest revision as of 14:23, 8 October 2013
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘
