Difference between revisions of "UgtP"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 +
**pGP2571 (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 16:27, 13 August 2013

  • Description: UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of cell division

Gene name ugtP
Synonyms ypfP
Essential no
Product UDP-glucose diacylglycerol glucosyltransferase
Function synthesis of glycolipids and anchoring of lipoteichoic
acid, inhibition of FtsZ assembly
Gene expression levels in SubtiExpress: ugtP
Interactions involving this protein in SubtInteract: UgtP
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 43 kDa, 8.398
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours metA, cspD
Sequences Protein DNA DNA_with_flanks
Genetic context
UgtP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
UgtP expression.png



















Categories containing this gene/protein

cell division, lipid metabolism/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU21920

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)
    • the interaction with FtsZ results in inhibition of cell division and an increase of cell size PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • oligomerization of UgtP is inhibited by UDP-Glc and by interaction with FtsZ PubMed
  • Localization:
    • membrane-bound protein, self-assembles into tightly wound spirals in vitro PubMed
    • under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with FtsZ PubMed
    • under nutrient poor conditions: forms punctate foci (oligomers), no interaction with FtsZ PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original Publications