IspE

• Description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispE
Synonyms	yabH , ipk
Essential	yes PubMed
Product	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispE
MW, pI	31 kDa, 6.349
Gene length, protein length	867 bp, 289 aa
Immediate neighbours	sspF, purR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

The gene

Basic information

• Locus tag: BSU00460

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)

• Protein family: IspE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1UEK (from E. coli, 38% identity) PubMed

• UniProt: P37550

• KEGG entry: [2]

• E.C. number: 2.7.1.148

Expression and regulation

• Operon:

• Expression browser: ispE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Takashi Wada, Tomohisa Kuzuyama, Shinya Satoh, Seiki Kuramitsu, Shigeyuki Yokoyama, Satoru Unzai, Jeremy R H Tame, Sam-Yong Park
Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis.
J Biol Chem: 2003, 278(32);30022-7
[PubMed:12771135] [WorldCat.org] [DOI] (P p)