Dxs

• Description: 1-deoxyxylulose-5-phosphate synthase, first step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	dxs
Synonyms	yqiE
Essential	yes PubMed
Product	1-deoxyxylulose-5-phosphate synthase
Function	biosynthesis of thiamine, MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: dxs
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	69 kDa, 6.002
Gene length, protein length	1899 bp, 633 aa
Immediate neighbours	yqxC, yqiD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU24270

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO₂ (according to Swiss-Prot)

• Protein family: DXPS subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2O1S (from E. coli, 44% identity, 62% similarity) PubMed

• UniProt: P54523

• KEGG entry: [2]

• E.C. number: 2.2.1.7

Expression and regulation

• Operon:

• Expression browser: dxs PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)