Difference between revisions of "DgcW"
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Revision as of 13:06, 16 May 2013
- Description: potential diguanylate cyclase and phosphodiesterase
| Gene name | ykoW |
| Synonyms | |
| Essential | no |
| Product | potential diguanylate cyclase and phosphodiesterase |
| Function | unknown |
| Gene expression levels in SubtiExpress: ykoW | |
| MW, pI | 84 kDa, 6.823 |
| Gene length, protein length | 2247 bp, 749 aa |
| Immediate neighbours | ykoV, ykoX |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13420
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- contains a central GGDEF domain and a C-terminal EAL domain PubMed
- aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O34311
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP850 (ykoW::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967]
[WorldCat.org]
[DOI]
(I p)
Kazushi Suzuki, Paul Babitzke, Sidney R Kushner, Tony Romeo
Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.
Genes Dev: 2006, 20(18);2605-17
[PubMed:16980588]
[WorldCat.org]
[DOI]
(P p)
M Y Galperin, T A Gaidenko, A Y Mulkidjanian, M Nakano, C W Price
MHYT, a new integral membrane sensor domain.
FEMS Microbiol Lett: 2001, 205(1);17-23
[PubMed:11728710]
[WorldCat.org]
[DOI]
(P p)
