Difference between revisions of "Mfd"
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| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mfd_60430_63963_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mfd_expression.png|500px]] | + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mfd_60430_63963_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mfd_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00550]] |
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Revision as of 13:19, 16 May 2013
- Description: transcription-repair coupling factor
| Gene name | mfd |
| Synonyms | |
| Essential | no |
| Product | transcription-repair coupling factor |
| Function | promotes strand-specific DNA repair by displacing
RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site |
| Gene expression levels in SubtiExpress: mfd
RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site | |
| Interactions involving this protein in SubtInteract: Mfd | |
| MW, pI | 133 kDa, 5.367 |
| Gene length, protein length | 3531 bp, 1177 aa |
| Immediate neighbours | fin, spoVT |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, transcription
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00550
Phenotypes of a mutant
In an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed. PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- promotes strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
- is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for CcpA PubMed and CodY PubMed)
- Protein family:
- Paralogous protein(s): RecG
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P37474
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1167 (del ermC), available in Stülke lab
- Expression vector:
- lacZ fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Articles
Additional publications: PubMed
