Difference between revisions of "AnsR"

From SubtiWiki
Jump to: navigation, search
(The AnsR regulon:)
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || negative regulation of the ''[[ansA]]-[[ansB]]'' operon
 
|style="background:#ABCDEF;" align="center"|'''Function''' || negative regulation of the ''[[ansA]]-[[ansB]]'' operon
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23590 ansR]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23590 ansR]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]'''
Line 24: Line 24:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansA]]'', ''[[yqxK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansA]]'', ''[[yqxK]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14291&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23590 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23590 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23590 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ansR_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ansR_context.gif]]

Revision as of 14:13, 13 May 2013

  • Description: transcription repressor of the ansA-ansB operon

Gene name ansR
Synonyms
Essential no
Product transcriptional regulator (Xre family)
Function negative regulation of the ansA-ansB operon
Gene expression levels in SubtiExpress: ansR
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 13 kDa, 4.807
Gene length, protein length 348 bp, 116 aa
Immediate neighbours ansA, yqxK
Sequences Protein DNA Advanced_DNA
Genetic context
AnsR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsR expression.png




























Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

AnsR regulon

The AnsR regulon:

The gene

Basic information

  • Locus tag: BSU23590

Phenotypes of a mutant

  • the ansR mutation allows glutamate utilization of a rocG gudB mutant PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1152 (del tet) available in the Stülke lab
  • Expression vector:
    • expression of native ansR in B. subtilis: pGP873 (in pBQ200), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References