Difference between revisions of "SspC"
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|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA | |style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19950 sspC] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 9.102 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 9.102 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yotB]]'', ''[[yosX]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yotB]]'', ''[[yosX]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19950 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19950 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19950 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:sspC_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:sspC_context.gif]] | ||
Revision as of 13:02, 13 May 2013
- Description: small acid-soluble spore protein (minor alpha/beta-type SASP)
| Gene name | sspC |
| Synonyms | |
| Essential | no |
| Product | small acid-soluble spore protein (minor alpha/beta-type SASP) |
| Function | protection of spore DNA |
| Gene expression levels in SubtiExpress: sspC | |
| MW, pI | 7 kDa, 9.102 |
| Gene length, protein length | 216 bp, 72 aa |
| Immediate neighbours | yotB, yosX |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
sporulation proteins, SP-beta prophage
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU19950
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 2Z3X (in complex with DNA)
- UniProt: P02958
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: sspC PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ki Seog Lee, Daniela Bumbaca, Jeffrey Kosman, Peter Setlow, Mark J Jedrzejas
Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species.
Proc Natl Acad Sci U S A: 2008, 105(8);2806-11
[PubMed:18287075]
[WorldCat.org]
[DOI]
(I p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Daphna Frenkiel-Krispin, Rinat Sack, Joseph Englander, Eyal Shimoni, Miriam Eisenstein, Esther Bullitt, Rachel Horowitz-Scherer, Christopher S Hayes, Peter Setlow, Abraham Minsky, Sharon Grayer Wolf
Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores.
J Bacteriol: 2004, 186(11);3525-30
[PubMed:15150240]
[WorldCat.org]
[DOI]
(P p)
Jeffrey Kosman, Peter Setlow
Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA.
J Bacteriol: 2003, 185(20);6095-103
[PubMed:14526021]
[WorldCat.org]
[DOI]
(P p)
Anjum Sohail, Christopher S Hayes, Pradeep Divvela, Peter Setlow, Ashok S Bhagwat
Protection of DNA by alpha/beta-type small, acid-soluble proteins from Bacillus subtilis spores against cytosine deamination.
Biochemistry: 2002, 41(38);11325-30
[PubMed:12234173]
[WorldCat.org]
[DOI]
(P p)
C S Hayes, P Setlow
An alpha/beta-type, small, acid-soluble spore protein which has very high affinity for DNA prevents outgrowth of Bacillus subtilis spores.
J Bacteriol: 2001, 183(8);2662-6
[PubMed:11274127]
[WorldCat.org]
[DOI]
(P p)
C S Hayes, E Alarcon-Hernandez, P Setlow
N-terminal amino acid residues mediate protein-protein interactions between DNA-bound alpha /beta -type small, acid-soluble spore proteins from Bacillus species.
J Biol Chem: 2001, 276(3);2267-75
[PubMed:11044450]
[WorldCat.org]
[DOI]
(P p)
C S Hayes, B Illades-Aguiar, L Casillas-Martinez, P Setlow
In vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species.
J Bacteriol: 1998, 180(10);2694-700
[PubMed:9573155]
[WorldCat.org]
[DOI]
(P p)
Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212]
[WorldCat.org]
[DOI]
(P p)
W L Nicholson, D X Sun, B Setlow, P Setlow
Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters.
J Bacteriol: 1989, 171(5);2708-18
[PubMed:2468649]
[WorldCat.org]
[DOI]
(P p)
M J Connors, P Setlow
Cloning of a small, acid-soluble spore protein gene from Bacillus subtilis and determination of its complete nucleotide sequence.
J Bacteriol: 1985, 161(1);333-9
[PubMed:2981806]
[WorldCat.org]
[DOI]
(P p)
