Difference between revisions of "YmcB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17010 ymcB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17010 ymcB]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 6.873   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 6.873   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kbl]]'', ''[[ymcA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kbl]]'', ''[[ymcA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13574&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17010 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17010 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17010 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:YmcB_context.png]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:YmcB_context.png]]

Revision as of 12:53, 13 May 2013

  • Description: tRNA methylthiotransferase

Gene name ymcB
Synonyms
Essential no
Product tRNA methylthiotransferase
Function tRNA maturation
Gene expression levels in SubtiExpress: ymcB
MW, pI 57 kDa, 6.873
Gene length, protein length 1527 bp, 509 aa
Immediate neighbours kbl, ymcA
Sequences Protein DNA Advanced_DNA
Genetic context
YmcB context.png
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YmcB expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU17010

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA PubMed
  • Protein family: MiaB subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YqeV

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1109 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References