Difference between revisions of "YmdB"
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=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' | + | * '''Mutant:''' GP583 (spc), available in [[Stülke]] lab |
− | * '''Expression vector:''' | + | * '''Expression vector:''' pGP1040 (N-termnial His-tag, in [[pWH844]]), pGP1041 (N-terminal Strep-tag, for SPINE, expression in ''B. subtilis'', in [[pGP382]]), available in [[Stülke]] lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' |
Revision as of 20:32, 14 January 2009
- Description: unknown function
Gene name | ymdB |
Synonyms | |
Essential | no |
Product | YmdB protein |
Function | unknown function |
MW, pI | 29,1 kDa, 6.50 |
Gene length, protein length | 792 bp, 264 amino acids |
Immediate neighbours | ymdA, spoVS |
Gene sequence (+200bp) | Protein sequence |
Genetic context |
Contents
The gene
Basic information
- Coordinates: 1768211 - 1769002
Phenotypes of a mutant
strong overexpression of Hag
Database entries
- DBTBS entry:
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: negative effector of the expression of hag and other members of the SigD regulon
- Protein family:
- Paralogous protein(s): similar to unknown proteins
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: [2]
- KEGG entry: [3]
- E.C. number:
Additional information
has a negative effect on hag expression
Expression and regulation
- Sigma factor:
- Regulation: constitutive
- Regulatory mechanism:
- Additional information: there is a terminator between rny and ymdB, most transcripts terminate there
Biological materials
- Mutant: GP583 (spc), available in Stülke lab
- Expression vector: pGP1040 (N-termnial His-tag, in pWH844), pGP1041 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP382), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed