Difference between revisions of "DgcW"
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykoW_1407329_1409578_-1 ykoW] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykoW_1407329_1409578_-1 ykoW] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP850 (''[[ykoW]]''::''cat''), available in [[Stülke]] lab | + | ** GP850 (''[[ykoW]]''::''cat''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
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=References= | =References= | ||
| − | + | <pubmed>11728710,16980588 , 22821967</pubmed> | |
| − | <pubmed>11728710,16980588 , </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 08:05, 19 April 2013
- Description: potential diguanylate cyclase and phosphodiesterase
| Gene name | ykoW |
| Synonyms | |
| Essential | no |
| Product | potential diguanylate cyclase and phosphodiesterase |
| Function | unknown |
| Gene expression levels in SubtiExpress: ykoW | |
| MW, pI | 84 kDa, 6.823 |
| Gene length, protein length | 2247 bp, 749 aa |
| Immediate neighbours | ykoV, ykoX |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13420
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- contains a central GGDEF domain and a C-terminal EAL domain PubMed
- aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O34311
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP850 (ykoW::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
