Difference between revisions of "PGP172"
| Line 18: | Line 18: | ||
**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | **'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | ||
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
| + | |||
| + | * important: EcoRI, although mentioned in the MCS, is not unique! | ||
| + | |||
<pubmed> 14717704 </pubmed> | <pubmed> 14717704 </pubmed> | ||
Revision as of 06:55, 16 April 2013
- vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
- host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
- constructed in the Stülke lab
- in E. coli: ampicillin resistance
- based on pET3C
- sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
- important: EcoRI, although mentioned in the MCS, is not unique!
