Difference between revisions of "FbaA"
| Line 8: | Line 8: | ||
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fba, fba1, tsr '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fba, fba1, tsr '' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || | + | |style="background:#ABCDEF;" align="center"| '''Essential''' || no |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || fructose-1,6-bisphosphate aldolase | |style="background:#ABCDEF;" align="center"| '''Product''' || fructose-1,6-bisphosphate aldolase | ||
| Line 37: | Line 37: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | + | <br/><br/> | |
| Line 54: | Line 54: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519}} | |
| − | * | ||
=== Database entries === | === Database entries === | ||
| Line 117: | Line 116: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fbaA_3808512_3809369_-1 fbaA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fbaA_3808512_3809369_-1 fbaA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 131: | Line 130: | ||
* '''Mutant:''' | * '''Mutant:''' | ||
| − | * GP591 (''fbaA''::''cat''), available in [[Stülke]] lab | + | * GP591 (''fbaA''::''cat''), available in [[Jörg Stülke]]'s lab |
| − | * GP596 (''fbaA''::''erm''), available in [[Stülke]] lab | + | * GP596 (''fbaA''::''erm''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1423, available in [[Stülke]] lab | + | ** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1423, available in [[Jörg Stülke]]'s lab |
| − | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP88, available in [[Stülke]] lab | + | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP88, available in [[Jörg Stülke]]'s lab |
| − | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP395, available in [[Stülke]] lab | + | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP395, available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' pGP601 (in [[pAC6]]) | * '''lacZ fusion:''' pGP601 (in [[pAC6]]) | ||
| Line 143: | Line 142: | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
| Line 152: | Line 151: | ||
=References= | =References= | ||
| − | + | <pubmed>17218307, 15125960, 24624 16843441 11489127 20525796 23420519 23033921</pubmed> | |
| − | <pubmed>17218307, 15125960, 24624 16843441 11489127 20525796</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:42, 14 April 2013
- Description: fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme
| Gene name | fbaA |
| Synonyms | fba, fba1, tsr |
| Essential | no |
| Product | fructose-1,6-bisphosphate aldolase |
| Function | enzyme in glycolysis/ gluconeogenesis |
| Gene expression levels in SubtiExpress: fbaA | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
| MW, pI | 30,2 kDa, 5.03 |
| Gene length, protein length | 855 bp, 285 amino acids |
| Immediate neighbours | ywjH, spo0F |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)
- Protein family: class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
- Paralogous protein(s): FbaB
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
- Modification: phosphorylation on Thr-212 and Thr-234 PubMed
- Cofactor(s): Zn2+ (Metalloenzyme)
- Effectors of protein activity:
Database entries
- Structure: 3Q94 (from Bacillus anthracis)
- UniProt: P13243
- KEGG entry: [3]
- E.C. number: 4.1.2.13
Additional information
- Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
- extensive information on the structure and enzymatic properties of FbaA can be found at Proteopedia
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
Biological materials
- Mutant:
- GP591 (fbaA::cat), available in Jörg Stülke's lab
- GP596 (fbaA::erm), available in Jörg Stülke's lab
- Expression vector:
- for expression in B. subtilis, in pBQ200: pGP1423, available in Jörg Stülke's lab
- for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP88, available in Jörg Stülke's lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP395, available in Jörg Stülke's lab
- lacZ fusion: pGP601 (in pAC6)
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
