Difference between revisions of "GndA"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
+ | ** pGP1777 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Stülke]] lab) | ||
** pGP1789 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP1389]]) (available in [[Stülke]] lab) | ** pGP1789 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP1389]]) (available in [[Stülke]] lab) | ||
** GP1408 (''gndA''-''Strep'' ''(spc)'') & GP1410 (''gndA''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [[SPINE]], available in [[Stülke]] lab | ** GP1408 (''gndA''-''Strep'' ''(spc)'') & GP1410 (''gndA''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [[SPINE]], available in [[Stülke]] lab |
Revision as of 08:42, 2 November 2012
- Description: NADP-dependent phosphogluconate dehydrogenase
Gene name | gndA |
Synonyms | yqjI |
Essential | no |
Product | NADP-dependent phosphogluconate dehydrogenase |
Function | pentose phosphate pathway |
Gene expression levels in SubtiExpress: gndA | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 51 kDa, 5.076 |
Gene length, protein length | 1407 bp, 469 aa |
Immediate neighbours | zwf, polY1 |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23860
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH (according to Swiss-Prot)
- Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible reaction PubMed
- Domains:
- Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P80859
- KEGG entry: [3]
- E.C. number: 1.1.1.44
Additional information
It contains a cysteine on the active site. The enzyme is a dimer. PubMed
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1514 (gndA::kan), available in Stülke lab
- Expression vector:
- pGP1777 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Stülke lab)
- pGP1789 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP1389) (available in Stülke lab)
- GP1408 (gndA-Strep (spc)) & GP1410 (gndA-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
- FLAG-tag construct:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed