Difference between revisions of "TsaD"
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| − | * '''Description:''' | + | * '''Description:''' required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with [[TsaC]]), [[universally conserved protein]] <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''tsaD'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydiE '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydiE, gcp '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | ||
| Line 13: | Line 13: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05940 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05940 tsaD] |
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/ | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/TsaD TsaD] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.016 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.016 | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
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=== Additional information=== | === Additional information=== | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| − | ** biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs {{PubMed|21183954 | + | ** biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([[TsaB]]-[[TsaD]]-[[TsaE]]) {{PubMed|23072323,21183954}} |
| − | |||
| − | * '''Protein family:''' | + | * '''Protein family:''' |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| − | ** | + | ** [[TsaB]]-[[TsaD]] {{PubMed|23072323,21285948}} |
| − | ** [[ | + | ** [[TsaD]]-[[TsaE]] {{PubMed|23072323}} |
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 146: | Line 139: | ||
=References= | =References= | ||
| + | <pubmed> 23072323 </pubmed> | ||
==Publications on the corresponding'' E. coli'' protein, YgjD== | ==Publications on the corresponding'' E. coli'' protein, YgjD== | ||
<pubmed> 21183954 19376873 20824107 21619589 21285948 </pubmed> | <pubmed> 21183954 19376873 20824107 21619589 21285948 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:13, 18 October 2012
- Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
| Gene name | tsaD |
| Synonyms | ydiE, gcp |
| Essential | yes PubMed |
| Product | tRNA modification enzyme |
| Function | tRNA modification |
| Gene expression levels in SubtiExpress: tsaD | |
| Interactions involving this protein in SubtInteract: TsaD | |
| MW, pI | 36 kDa, 5.016 |
| Gene length, protein length | 1038 bp, 346 aa |
| Immediate neighbours | ydiD, ydiF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, universally conserved proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU05940
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O05518
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Charles T Lauhon
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP.
Biochemistry: 2012, 51(44);8950-63
[PubMed:23072323]
[WorldCat.org]
[DOI]
(I p)
Publications on the corresponding E. coli protein, YgjD
Tobias Bergmiller, Rafael Peña-Miller, Alexander Boehm, Martin Ackermann
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
BMC Microbiol: 2011, 11;118
[PubMed:21619589]
[WorldCat.org]
[DOI]
(I e)
Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948]
[WorldCat.org]
[DOI]
(I p)
Madhusudhan Srinivasan, Preeti Mehta, Yao Yu, Evelyn Prugar, Eugene V Koonin, A Wali Karzai, Rolf Sternglanz
The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A.
EMBO J: 2011, 30(5);873-81
[PubMed:21183954]
[WorldCat.org]
[DOI]
(I p)
Chen Katz, Ifat Cohen-Or, Uri Gophna, Eliora Z Ron
The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
mBio: 2010, 1(3);
[PubMed:20824107]
[WorldCat.org]
[DOI]
(I e)
Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873]
[WorldCat.org]
[DOI]
(I p)
