Difference between revisions of "TsaE"
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| − | * '''Description:''' P-loop ATPase <br/><br/> | + | * '''Description:''' P-loop ATPase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || tRNA modification enzyme |
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05910 ydiB] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05910 ydiB] | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[translation]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([[TsaB]]-[[TsaD]]-[[TsaE]]) {{PubMed|23072323,21183954}} | ||
* '''Protein family:''' UPF0079 family (according to Swiss-Prot) | * '''Protein family:''' UPF0079 family (according to Swiss-Prot) | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
** dimerizes and oligomerizes [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed] | ** dimerizes and oligomerizes [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed] | ||
| − | ** | + | ** [[TsaB]]-[[TsaE]] {{PubMed|23072323}} |
| + | ** [[TsaD]]-[[TsaE]] {{PubMed|23072323}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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=References= | =References= | ||
| − | <pubmed> 17005971, 19246765 </pubmed> | + | <pubmed> 17005971, 19246765 23072323</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:12, 18 October 2012
- Description: P-loop ATPase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA
| Gene name | ydiB |
| Synonyms | |
| Essential | no |
| Product | tRNA modification enzyme |
| Function | tRNA modification |
| Gene expression levels in SubtiExpress: ydiB | |
| Interactions involving this protein in SubtInteract: YdiB | |
| MW, pI | 17 kDa, 4.597 |
| Gene length, protein length | 474 bp, 158 aa |
| Immediate neighbours | thiL, ydiC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU05910
Phenotypes of a mutant
reduced growth rate PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0079 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: K(M) for ATP: 60 myM, V(max) 10 nmol/min PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm with the excepton of the nucleoid region PubMed
Database entries
- Structure:
- UniProt: O05515
- KEGG entry: [2]
- E.C. number:
Additional information
ATPase activity is required for in vivo function of the protein PubMed
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
