Difference between revisions of "MleN"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | + | {{SubtiWiki regulon|[[CcpA regulon]]}} | |
=The gene= | =The gene= | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** repressed by glucose ([[CcpA]]) {{PubMed|22900538}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[CcpA]]: transcription repression {{PubMed|22900538}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | <pubmed>18763711, 10903309 </pubmed> | + | <pubmed>18763711, 10903309 22900538</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:57, 23 August 2012
- Description: malate-H+/Na+-lactate antiporter
| Gene name | mleN |
| Synonyms | yqkI |
| Essential | no |
| Product | malate-H+/Na+-lactate antiporter |
| Function | malate uptake |
| Gene expression levels in SubtiExpress: mleN | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 50 kDa, 7.249 |
| Gene length, protein length | 1404 bp, 468 aa |
| Immediate neighbours | mleA, ansB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
transporters/ other, utilization of specific carbon sources, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23560
Phenotypes of a mutant
abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)
- Paralogous protein(s): NhaC
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- membrane associated PubMed
Database entries
- Structure:
- UniProt: P54571
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538]
[WorldCat.org]
[DOI]
(I e)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
Y Wei, A A Guffanti, M Ito, T A Krulwich
Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that enhances growth on malate at low protonmotive force.
J Biol Chem: 2000, 275(39);30287-92
[PubMed:10903309]
[WorldCat.org]
[DOI]
(P p)
