ThyA

• Description: thymidylate synthase A
  
  

Gene name	thyA
Synonyms
Essential	no
Product	thymidylate synthase A
Function	biosynthesis of thymidine nucleotides
Gene expression levels in SubtiExpress: thyA
MW, pI	32 kDa, 5.502
Gene length, protein length	837 bp, 279 aa
Immediate neighbours	cotU, yncM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

The gene

Basic information

• Locus tag: BSU17680

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP (according to Swiss-Prot)

• Protein family: Bacterial-type thyA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1B02

• UniProt: P42326

• KEGG entry: [2]

• E.C. number: 2.1.1.45

Expression and regulation

• Operon:

• Expression browser: thyA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

A Yazgan, G Ozcengiz, M A Marahiel
Tn10 insertional mutations of Bacillus subtilis that block the biosynthesis of bacilysin.
Biochim Biophys Acta: 2001, 1518(1-2);87-94
[PubMed:11267663] [WorldCat.org] [DOI] (P p)