Difference between revisions of "PGP1459"
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[[File:PGP1459.png|250px|thumb|right|'''PGP1459: click to enlarge''']] | [[File:PGP1459.png|250px|thumb|right|'''PGP1459: click to enlarge''']] | ||
| + | [[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']] | ||
The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP380]] it can be used for the [[SPINE]] method. | The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP380]] it can be used for the [[SPINE]] method. | ||
| − | + | The cloning region of [[pGP380]] is shown for detailed information. | |
| + | |||
Sequencing primers: | Sequencing primers: | ||
Revision as of 12:30, 16 May 2012
The vector was constructed in the Stülke lab and it is suitable for constitutive expression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. Just like pGP380 it can be used for the SPINE method.
The cloning region of pGP380 is shown for detailed information.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Similar plasmid: pGP1460 (for C-terminal Strep-tags)
