YjiB

• Description: cytochrome P450, monooxygenase CYP109B1
  
  

Gene name	yjiB
Synonyms
Essential	no
Product	monooxygenase CYP109B1
Function	oxidation of fatty acids
Interactions involving this protein in SubtInteract: YjiB
MW, pI	44 kDa, 5.379
Gene length, protein length	1188 bp, 396 aa
Immediate neighbours	yjiA, yjiC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other

The gene

Basic information

• Locus tag: BSU12210

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group PubMed

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YjiB-YkuN PubMed, YjiB-YkuP PubMed

• Localization:

Database entries

• Structure:

• UniProt: O34374

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yjiB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marco Girhard, Tobias Klaus, Yogan Khatri, Rita Bernhardt, Vlada B Urlacher
Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 87(2);595-607
[PubMed:20186410] [WorldCat.org] [DOI] (I p)

Marco Girhard, Kazuhiro Machida, Masashi Itoh, Rolf D Schmid, Akira Arisawa, Vlada B Urlacher
Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system.
Microb Cell Fact: 2009, 8;36
[PubMed:19591681] [WorldCat.org] [DOI] (I e)