YycI

• Description: negative effector of WalK, controls cell wall metabolism
  
  

Gene name	yycI
Synonyms
Essential	no
Product	negative effector of WalK
Function	control of cell wall metabolism
Interactions involving this protein in SubtInteract: YycI
MW, pI	32 kDa, 5.279
Gene length, protein length	840 bp, 280 aa
Immediate neighbours	walJ, yycH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall/ other, transcription factors and their control, membrane proteins

The gene

Basic information

• Locus tag: BSU40380

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers
  • YycH-YycI PubMed
  • WalK-YycI PubMed

• Localization: cell membrane (according to Swiss-Prot), spotty close to the membrane PubMed

Database entries

• Structure: 2O3O, PubMed

• UniProt: Q45612

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-walJ-yyxA PubMed

• Expression browser: yycI PubMed

• Sigma factor:

• Regulation: expressed during vegetative growth, repressed during stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tatsuya Fukushima, Isako Furihata, Robyn Emmins, Richard A Daniel, James A Hoch, Hendrik Szurmant
A role for the essential YycG sensor histidine kinase in sensing cell division.
Mol Microbiol: 2011, 79(2);503-22
[PubMed:21219466] [WorldCat.org] [DOI] (I p)

Hendrik Szurmant, Lintao Bu, Charles L Brooks, James A Hoch
An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteins.
Proc Natl Acad Sci U S A: 2008, 105(15);5891-6
[PubMed:18408157] [WorldCat.org] [DOI] (I p)

Patrick D McLaughlin, Benjamin G Bobay, Erin J Regel, Richele J Thompson, James A Hoch, John Cavanagh
Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition.
FEBS Lett: 2007, 581(7);1425-9
[PubMed:17350627] [WorldCat.org] [DOI] (P p)

Hendrik Szurmant, Michael A Mohan, P Michael Imus, James A Hoch
YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis.
J Bacteriol: 2007, 189(8);3280-9
[PubMed:17307850] [WorldCat.org] [DOI] (P p)

Eugenio Santelli, Robert C Liddington, Michael A Mohan, James A Hoch, Hendrik Szurmant
The crystal structure of Bacillus subtilis YycI reveals a common fold for two members of an unusual class of sensor histidine kinase regulatory proteins.
J Bacteriol: 2007, 189(8);3290-5
[PubMed:17307848] [WorldCat.org] [DOI] (P p)

C Fabret, J A Hoch
A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy.
J Bacteriol: 1998, 180(23);6375-83
[PubMed:9829949] [WorldCat.org] [DOI] (P p)