Difference between revisions of "Methods"

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(Genetic work with non-standard strains)
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* transformation of wild-type ''B. subtilis'' strains incl. NCIB3610: {{PubMed|20300532}}
 
* transformation of wild-type ''B. subtilis'' strains incl. NCIB3610: {{PubMed|20300532}}
 
* efficient electroporation of ''B. subtilis'' {{PubMed|22387342}}
 
* efficient electroporation of ''B. subtilis'' {{PubMed|22387342}}
 +
* a novel transformation protocol for ''B. subtilis'' DB104 {{PubMed|22395911}}
  
 
==General procedures==
 
==General procedures==

Revision as of 11:58, 8 March 2012

Here you can find a list of methods for working with Bacillus subtilis:

Analysis of protein-protein interactions

  • Bacterial Two Hybrid: BACTH

Analysis of membrane proteins

  • application of a split green fluorescent protein reporter PubMed

Strain construction

  • introduction of markerfree deletions: PubMed

RNA analysis

  • Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf

Genetic work with non-standard strains

  • transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
  • efficient electroporation of B. subtilis PubMed
  • a novel transformation protocol for B. subtilis DB104 PubMed

General procedures

  • Standard operation procedures (SOPs) of the SYSMO-BACELL consortium

Live cell imaging

  • Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
  • Assay of gene expression dynamics using live cell imaging PubMed

Transposon mutagenesis

  • mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed

Protein-DNA interactions

  • ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed

Key references

Additional publications: PubMed