Nin

• Description: inhibitor of the DNA degrading activity of NucA
  
  

Gene name	nin
Synonyms	comJ
Essential	no
Product	inhibitor of the DNA degrading activity of NucA
Function	genetic transformation, DNA uptake
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	14 kDa, 4.922
Gene length, protein length	396 bp, 132 aa
Immediate neighbours	yckE, nucA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU03420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P12669

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nucA-nin PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism: ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

R Provvedi, I Chen, D Dubnau
NucA is required for DNA cleavage during transformation of Bacillus subtilis.
Mol Microbiol: 2001, 40(3);634-44
[PubMed:11359569] [WorldCat.org] [DOI] (P p)

D van Sinderen, R Kiewiet, G Venema
Differential expression of two closely related deoxyribonuclease genes, nucA and nucB, in Bacillus subtilis.
Mol Microbiol: 1995, 15(2);213-23
[PubMed:7746143] [WorldCat.org] [DOI] (P p)