Difference between revisions of "PGP888"
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Revision as of 11:44, 26 August 2011
The vector was constructed in the Stülke lab and it is suitable for the xylose-inducible expression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI or NotI
Hints for usage
- An additional base has to be inserted in the forward primer to restore the frame.
- Don't forget a stop codon in the reverse primer.
The reference
