UvrB

• Description: excinuclease ABC (subunit B)
  
  

Gene name	uvrB
Synonyms	dinA, uvrA
Essential	no
Product	excinuclease ABC (subunit B)
Function	DNA repair after UV damage
Interactions involving this protein in SubtInteract: UvrB
MW, pI	76 kDa, 5.26
Gene length, protein length	1983 bp, 661 aa
Immediate neighbours	uvrA, csbA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU35170

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: uvrB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • UvrA-UvrB PubMed
  • UvrB-UvrC PubMed
  • UvrB interacts sequentially with UvrA and UvrC, a complex of the three proteins was never observed PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2NMV (bound to fluorescein-adducted DNA); 2D7D ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP PubMed

• UniProt: P37954

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: uvrB-uvrA (according to DBTBS)

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1175 (del uvrAB::ermC) (available in the Stülke lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004] [WorldCat.org] [DOI] (P p)

Bennett Van Houten, Deborah L Croteau, Matthew J DellaVecchia, Hong Wang, Caroline Kisker
'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system.
Mutat Res: 2005, 577(1-2);92-117
[PubMed:15927210] [WorldCat.org] [DOI] (P p)

A Sancar
Mechanisms of DNA excision repair.
Science: 1994, 266(5193);1954-6
[PubMed:7801120] [WorldCat.org] [DOI] (P p)

Original Publications

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Jitka Eryilmaz, Simona Ceschini, James Ryan, Stella Geddes, Timothy R Waters, Tracey E Barrett
Structural insights into the cryptic DNA-dependent ATPase activity of UvrB.
J Mol Biol: 2006, 357(1);62-72
[PubMed:16426634] [WorldCat.org] [DOI] (P p)

K W Winterling, D Chafin, J J Hayes, J Sun, A S Levine, R E Yasbin, R Woodgate
The Bacillus subtilis DinR binding site: redefinition of the consensus sequence.
J Bacteriol: 1998, 180(8);2201-11
[PubMed:9555905] [WorldCat.org] [DOI] (P p)

C M Lovett, K C Cho, T M O'Gara
Purification of an SOS repressor from Bacillus subtilis.
J Bacteriol: 1993, 175(21);6842-9
[PubMed:8226626] [WorldCat.org] [DOI] (P p)