Difference between revisions of "YwkE"
| Line 82: | Line 82: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=1NV8 1NV8] (the protein from ''Thermotoga maritima'') {{PubMed|12741815}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P45873 P45873] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P45873 P45873] | ||
| Line 123: | Line 123: | ||
=References= | =References= | ||
| − | <pubmed> 11805295 11847124 </pubmed> | + | <pubmed> 11805295 11847124 12741815 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:48, 1 August 2011
- Description: similar to N5-glutamine methyltransferase that modifies peptide release factors
| Gene name | ywkE |
| Synonyms | |
| Essential | no |
| Product | N5-glutamine methyltransferase |
| Function | peptide release factor methylation |
| MW, pI | 32 kDa, 4.89 |
| Gene length, protein length | 864 bp, 288 aa |
| Immediate neighbours | ywkF, prfA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
translation, protein modification
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37000
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: hemK family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: P45873
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Heidi L Schubert, John D Phillips, Christopher P Hill
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.
Biochemistry: 2003, 42(19);5592-9
[PubMed:12741815]
[WorldCat.org]
[DOI]
(P p)
Valérie Heurgué-Hamard, Stéphanie Champ, Ake Engström, Måns Ehrenberg, Richard H Buckingham
The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
EMBO J: 2002, 21(4);769-78
[PubMed:11847124]
[WorldCat.org]
[DOI]
(P p)
Kenji Nakahigashi, Naoko Kubo, Shin-ichiro Narita, Takeshi Shimaoka, Simon Goto, Taku Oshima, Hirotada Mori, Maki Maeda, Chieko Wada, Hachiro Inokuchi
HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
Proc Natl Acad Sci U S A: 2002, 99(3);1473-8
[PubMed:11805295]
[WorldCat.org]
[DOI]
(P p)
