SecY

• Description: preprotein translocase subunit, universally conserved protein
  
  

Gene name	secY
Synonyms
Essential	yes PubMed
Product	preprotein translocase subunit
Function	protein secretion
Interactions involving this protein in SubtInteract: SecY
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	47 kDa, 10.209
Gene length, protein length	1293 bp, 431 aa
Immediate neighbours	rplO, adk
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, essential genes, membrane proteins, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01360

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: secY/SEC61-alpha family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: (SecY-SecE-SecG)2-SecA2 PubMed

• Localization: membrane PubMed

Database entries

• Structure: 3DL8 (structure of the (SecY-SecE-SecG)-SecA complex PubMed

• UniProt: P16336

• KEGG entry: [2]

• E.C. number:

Additional information

universally conserved protein

Expression and regulation

• Operon: rplO-secY-adk-map

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: Pspac-secY PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jochen Zimmer, Yunsun Nam, Tom A Rapoport
Structure of a complex of the ATPase SecA and the protein-translocation channel.
Nature: 2008, 455(7215);936-43
[PubMed:18923516] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

John F Hunt, Sevil Weinkauf, Lisa Henry, John J Fak, Paul McNicholas, Donald B Oliver, Johann Deisenhofer
Nucleotide control of interdomain interactions in the conformational reaction cycle of SecA.
Science: 2002, 297(5589);2018-26
[PubMed:12242434] [WorldCat.org] [DOI] (I p)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)

R Breitling, B Schlott, D Behnke
Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis.
J Basic Microbiol: 1994, 34(3);145-55
[PubMed:8071801] [WorldCat.org] [DOI] (P p)

J W Suh, S A Boylan, S M Thomas, K M Dolan, D B Oliver, C W Price
Isolation of a secY homologue from Bacillus subtilis: evidence for a common protein export pathway in eubacteria.
Mol Microbiol: 1990, 4(2);305-14
[PubMed:2110998] [WorldCat.org] [DOI] (P p)