Difference between revisions of "SubtInteract"
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Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as [[DNA replication]], [[transcription]] or [[translation]]. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature. | Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as [[DNA replication]], [[transcription]] or [[translation]]. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature. | ||
| − | + | =Methods to detect protein-protein interactions= | |
* Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}} | * Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}} | ||
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'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}} | '''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}} | ||
| − | + | =Visualization of protein-protein interactions in ''B. subtilis''= | |
* [http://cellpublisher.gobics.de/subtinteract/startpage/start/ interactive protein-protein interaction map] | * [http://cellpublisher.gobics.de/subtinteract/startpage/start/ interactive protein-protein interaction map] | ||
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract] | * the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract] | ||
| − | + | =Protein complexes in ''B. subtilis''= | |
| + | ==Complexes in [[Cellular processes]]== | ||
| + | === [[cell wall synthesis]] and [[cell shape]]: the [[cell wall biosynthhetic complex]]=== | ||
| + | === [[cell division]]: the [[divisome]]=== | ||
| + | |||
| + | ==Complexes in [[Metabolism]]== | ||
| + | === the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | ||
| + | |||
| + | ==Complexes in [[Information processing]]== | ||
=== [[DNA replication]]: the [[replisome]]=== | === [[DNA replication]]: the [[replisome]]=== | ||
=== [[transcription]]: [[RNA polymerase]]=== | === [[transcription]]: [[RNA polymerase]]=== | ||
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=== synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]=== | === synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]=== | ||
=== [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]=== | === [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]=== | ||
| + | |||
| + | ==Complexes involved in [[Lifestyles]]== | ||
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]=== | === [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]=== | ||
| − | |||
=== DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | === DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | ||
| − | |||
===spore [[germination]]: the [[germinosome]]=== | ===spore [[germination]]: the [[germinosome]]=== | ||
Revision as of 18:40, 10 July 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Contents
- 1 Methods to detect protein-protein interactions
- 2 Visualization of protein-protein interactions in B. subtilis
- 3 Protein complexes in B. subtilis
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- interactive protein-protein interaction map
- the beta version of SubtInteract
Protein complexes in B. subtilis
Complexes in Cellular processes
cell wall synthesis and cell shape: the cell wall biosynthhetic complex
cell division: the divisome
Complexes in Metabolism
the metabolons of glycolysis and the TCA cycle PubMed
Complexes in Information processing
DNA replication: the replisome
transcription: RNA polymerase
translation: the ribosome
synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
RNA processing and degradation: the RNA degradosome
Complexes involved in Lifestyles
general stress response: the stressosome
DNA uptake: the pseudopilus PubMed
spore germination: the germinosome
Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)
- FruA, SwrC, XhlA, YhaP, YhgE, YkcC, YqfF, CsbC, CssS, FliZ, MreD, PpsC, RacA, Smc, YclI, YdeL, YdgH, YdbI, YesS, YkoT, YopZ, YqbD, YtdP, YueB, YwqJ, YyxA
Important publications
