Difference between revisions of "SubtInteract"

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(Protein complexes in B. subtilis)
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Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as [[DNA replication]], [[transcription]] or [[translation]]. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
 
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as [[DNA replication]], [[transcription]] or [[translation]]. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
  
==Methods to detect protein-protein interactions==
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=Methods to detect protein-protein interactions=
 
* Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}}
 
* Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}}
  
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'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
 
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
  
==Visualization of protein-protein interactions in ''B. subtilis''==
+
=Visualization of protein-protein interactions in ''B. subtilis''=
 
* [http://cellpublisher.gobics.de/subtinteract/startpage/start/ interactive protein-protein interaction map]  
 
* [http://cellpublisher.gobics.de/subtinteract/startpage/start/ interactive protein-protein interaction map]  
 
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]
 
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]
  
==Protein complexes in ''B. subtilis''==
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=Protein complexes in ''B. subtilis''=
 +
==Complexes in [[Cellular processes]]==
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=== [[cell wall synthesis]] and [[cell shape]]: the [[cell wall biosynthhetic complex]]===
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=== [[cell division]]: the [[divisome]]===
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 +
==Complexes in [[Metabolism]]==
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=== the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
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 +
==Complexes in [[Information processing]]==
 
=== [[DNA replication]]: the [[replisome]]===
 
=== [[DNA replication]]: the [[replisome]]===
 
=== [[transcription]]: [[RNA polymerase]]===
 
=== [[transcription]]: [[RNA polymerase]]===
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=== synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]===
 
=== synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]===
 
=== [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]===
 
=== [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]===
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 +
==Complexes involved in [[Lifestyles]]==
 
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]===
 
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]===
=== [[cell division]]: the [[divisome]]===
 
 
=== DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}===
 
=== DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}===
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
 
 
===spore [[germination]]: the [[germinosome]]===
 
===spore [[germination]]: the [[germinosome]]===
  

Revision as of 18:40, 10 July 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

Complexes in Cellular processes

cell wall synthesis and cell shape: the cell wall biosynthhetic complex

cell division: the divisome

Complexes in Metabolism

the metabolons of glycolysis and the TCA cycle PubMed

Complexes in Information processing

DNA replication: the replisome

transcription: RNA polymerase

translation: the ribosome

synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln

RNA processing and degradation: the RNA degradosome

Complexes involved in Lifestyles

general stress response: the stressosome

DNA uptake: the pseudopilus PubMed

spore germination: the germinosome

Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)


Important publications