Program

Sunday, June 19, 2011 – Afternoon

• 2:00 Welcome remarks – Jim Hoch

Physiology

CHAIR: Josef Deutscher

• 2:15 – 2:30 T1, Elaine Milohanic, INRA and AgroParis Tech, Thiverval-Grignon, France
  • ManR regulates the expression of the main Listeria monocytogenes glucose/mannose transporter and uses a second glucose transporter as substrate sensor.

• 2:30 – 2:45 T2, Boris Görke, Dept. of General Microbiology, Georg-August University of Göttingen, Göttingen, Germany
  • Crh, the homolog of the phosphor-carrier protein HPr, regulates a glycolytic bypass in Bacillus subtilis.

• 2:45 – 3:00 T3, Jean-Michel Jault, Institut de Biologie Structurale, Grenoble, France
  • The unusual activity of a GTPase from Bacillus subtilis, YphC (or EngA), involved in ribosome biogenesis.

• 3:00 – 3:15 T4, Yuka Kazo, Laboratory of Molecular Genetics, Department of Life Science, Rikkyo University, Tokyo, Japan
  • Analysis of dimerization of 70S ribosome, and yvyD gene during stationary phase in Bacillus subtilis.

• 3:15 – 3:30 T5, Donald A. Morrison, Dept. of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, USA
  • Regulation of the competence regulon in Group A and Group B streptococci: Pyogenic Type II ComR receptors recognize small species-specific peptide pheromones when expressed in a heterologous host.

• 3:30 – 3:45 T6, Nathalie Campo, Centre national de la Recherche Scientifique, Toulouse, France
  • X-state (competence) development causes transient cell cycle alterations in Streptococcus pneumoniae.

• 3:45 – 4:00 T7, Maria C. Mansilla, Instituto de Biología Molecular y Celular, Univ. Nac.de Rosario, Rosario, Argentina
  • A novel pathway for lipoic acid synthesis in Gram-positive bacteria.

• 4:00 – 4:15 T8, Jörg Stülke, Dept. of General Microbiology, Georg-August- Univeristy Göttingen, Göttingen, Germany
  • A novel factor controlling bistability in Bacillus subtilis: The YmdB protein affects biofilm formation and motility.

Genome Engineering

CHAIR: Mike Rasmussen

• 4:45 – 5:00 T9, Robert A. Britton, Dept. of Microbiology and Molecular Genetics, Michigan State University, East Lansing Michigan, USA
  • Recombineering in lactic acid bacteria.

• 5:00 – 5:15 T10, Ghislain Schyns, DSM Nutritional Products, Ltd., Basel, Switzerland
  • Learning from probiotics to improve biological availability of feed enzymes.

• 5:15 – 5:30 T11, Sébastien Potot, DSM Nutritional Products, Biotechnology, Basel, Switzerland
  • Mixing bacterial genomes to identify critical mutations in Bacillus subtilis industrial strains.

• 5:30 – 5:45 T12, Philippe Jacques, Université Lille, France
  • Environmental factors and pleiotropic regulators influence homologous compound pattern of mycosubtilin produced by Bacilius subtilis ATCC 6633.

• 5:45 – 6:00 T13, Philippe Noirot, INRA, Jouy en Josas, France
  • Systematic identification of dispensable genome parts in Bacillus subtilis coupled with metabolic model reconstruction.

Monday, June 20, 2011 – Morning

Cell Wall I

CHAIR: Kevin Devine

• 8:15 – 9:00, Jeff Errington, Centre for Bacterial Cell Biology, Newcastle University, Newcastle-upon-Tyne, United Kingdom
  • Keynote Lecture: Emergence and proliferation of B. subtilis L-forms.

• 9:00 – 9:15 T14, Olaf Schneewind, Dept. of Microbiology, University of Chicago, Chicago, Illinois, USA
  • LytN, a murein hydrolase in the cross wall compartment of Staphylococcus aureus, is required for bacterial growth and envelope assembly.

• 9:15 – 9:30 T15, Hèléne Bierne, Institut Pasteur, Unité des interactions bactéries-cellules, Inserm, Paris, France
  • A regulated switch from helical to polar localization of Listeria monocytogenes cell wall-anchored proteins.

• 9:30 – 9:45 T16 Sven Halbedel, Dept. of Bacterial Infections, Robert Koch Institute, Wernigerode, Germany
  • A role for the membrane curvature sensor DivIVA in cell separation and virulence of Listeria monocytogenes.

• 9:45 – 10:00 T17, Teresa Costa, ITQB, Universidade Nova de Lisboa, Oeiras, Portugal
  • Control of Bacillus subtilis cell shape by the novel cytoskeletal element RodZ.

• 10:00 – 10:15 T18, Julia Domínguez-Escobar, Max Planck Institute of Biochemistry, Martinsried, Germany
  • Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria. In vivo visualization of sidewall biosynthetic machineries in Bacillus subtilis.

• 10:15 – 10:30 T19, Anne Galinier, Université de la Méditerranée, Marseille, France
  • The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis.

Cell Wall II

CHAIR: Jeff Errington

• 11:00 – 11:15 T20, Letal Salzberg, Smurfit Institute of Genetics, Trintiy College Dublin, Dublin, Ireland
  • WalR-WalK (YycF-YycG)-mediated control of cell wall metabolism in Bacillus subtilis: linking autolysins and actin-like proteins.

• 11:15 – 11:30 T21, Hiraku Takada, Tokyo University of Agriculture, Tokyo, Japan
  • Involvement of essential lipid synthetic enzyme, PlsX, in the cell division machinery.

• 11:30 – 11:45 T22, Maria-Halima Laaberki, Dept. of Microbiology and Immunology, Columbia University, New York, New York, USA
  • The unexpected role of O-acetylation of peptidoglycan in cell separation and S-layer anchoring in Bacillus anthracis.

• 11:45 – 12:00 T23, Michel-Yves Mistou, INRA, Institut Micalis, Jouy-en-Josas, France
  • Outer polysaccharide pellicle: a structural component of Gram positive bacteria cell wall.

• 12:00 – 12:15 T24, Rick Lewis, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle-upon-Tyne, United Kingdom
  • The decoration of peptidoglycan by teichoic acids: the missing enzymatic step revealed by X-ray crystallography?

Monday, June 20, 2011 – Afternoon

Structure

CHAIR: Jim Hoch

• 2:00 – 2:15 T25, Wayne F. Anderson, Northwestern University CSGID, Chicago, Illinois USA
  • Applying structural genomics – revealing the bacterial surface one protein structure at a time.

• 2:15 – 2:30 T26, Catarina G. Fernandes, ITQB, Universidade Nova de Lisboa, Oeiras, Portugal
  • From the crystal structure of the Tgl transglutaminase to the cross-linking of the spore surface layers.

• 2:30 – 2:45 T27, Claes von Wachenfeldt, Dept. of Biology, Lund University, Lund, Sweden
  • Redox sensing by Rex family repressors.

• 2:45 – 3:00 T28, Marion Boudes, IBBMC, Université Paris-Sud, Orsay, France
  • Structural and functional study of ComE, a key actor in S. pneumoniae competence.

• 3:00 – 3:15 T29, Hendrik Szurmant, Dept. of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA
  • Sensor histidine kinase signaling: An active conformation deduced from integrating genomics, molecular dynamics and mutagenesis.

Sporulation/ germination

CHAIR: Charles Moran

• 3:45 – 4:00 T30, Michael Young, Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, United Kindom
  • Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum.

• 4:00 – 4:15 T31, Lei Shi, INRA-AgroParis Tech, Micalis Institute, Jouy-en-Josas, France
  • Phosphorylated bacterial recombinase checks chromosome integrity during spore formation.

• 4:15 – 4:30 T32, Patrick Piggot, Dept. Microbiol. & Immunol., Temple University School of Medicine, Philadelphia, Pennsylvania, USA
  • Compartmentalization of σE Activity During Sporulation of Bacillus subtilis: Much Ado About What?

• 4:30 – 4:45 T33, Adriano Henriques, ITQB, Universidade Nova de Lisboa, Oeiras, Portugal
  • A negative feedback loop that limits the ectopic activation of a cell type-specific sporulation sigma factor of Bacillus subtilis.

• 4:45 – 5:00 T34, Thierry Doan, Dept. of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, USA
  • Bacterial membrane fission during sporulation in Bacillus subtilis.

• 5:00 – 5:15 T35, Tony Wilkinson, Dept. of Chemistry, University of York, UK
  • Structural and Interaction Studies of an Intercellular Complex Mediating Engulfment during Sporulation in B. subtilis.

• 5:15 – 5:30 T36, Patrick Eichenberger, Center for Genomics and Systems Biology, Dept. of Biology, New York University, New York, USA
  • A Spatial and Temporal map of the Bacillus subtilis Spore Coat.

• 5:30 – 5:45 T37, Alex Ter Beek, Molecular Biology & Microbial Food Safety, University of Amsterdam, The Netherlands
  • Live-cell imaging of aerobic bacteria: a tool to assess and model heterogeneous germination & outgrowth of Bacillus subtilis spores.

Poster Session I

• 5:45 – 7:45, Palazzo dei Congressi Sala Alba

Tuesday, June 21, 2011– Morning

Pathogenic Sporeformers - Clostridia

CHAIR: Mike Young

• 8:30 – 9:15, Neil Fairweather, Centre for Molecular Microbiology and Infection, Dept. of Life Sciences, Imperial College London, London, UK
  • Keynote Lecture: The cell wall of Clostridium difficile – is variability a key to pathogenesis?

• 9:15 – 9:30 T38, Linc Sonenshein, Tufts University School of Medicine, Boston, Massachusetts, USA
  • Regulation of Clostridium difficile virulence.

• 9:30 – 9:45 T39, Zhen Zhang, Faculty of Veterinary Medicine, University of Helsinki, Finland
  • A Two-Component System Negatively Regulates Botulinum Neurotoxin Expression.

• 9:45 – 10:00 T40, Eric A. Johnson, University of Wisconsin, Madison, Wisconsin, USA
  • Identification, Purification and Characterization of Botulinum Neurotoxin BoNT/A Subtype Toxins.

• 10:00 – 10:15 T41, Stephen Melville, Dept. of Biological Sciences, Virginia Tech, Blacksburg, Virginia, USA
  • Spatial and temporal linkage of type IV pili assembly and division septum formation in Clostridium perfringens.

• 10:15 – 10:30 T42, Jon Marles-Wright, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle-upon-Tyne, UK
  • Towards an understanding of the anatomy of Clostridial bacterial microcompartments.

Pathogenic Sporeformers - Bacillus anthracis

CHAIR: Olaf Schneewind

• 11:00 – 11:15 T43, Theresa M. Koehler, Dept. Microbiol. and Molec. Genet., University of Texas Medical School, Houston, Texas, USA
  • Modulation of the Bacillus anthracis Secretome.

• 11:15 – 11:30 T44, Susanne Pohl, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle-upon-Tyne, UK
  • The secondary secretion pathway in Bacillus anthracis.

• 11:30 – 11:45 T45, Agnès Fouet, Toxines et Pathogénie Bactériennes, Institut Pasteur, Paris, France
  • Bacillus anthracis virulence regulator AtxA mediates bicarbonate induction of toxin gene expression.

• 11:45 – 12:00 T46, Colin Harwood, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle-upon-Tyne, UK
  • Are superoxide ions a key environmental signal for the Bacillus anthracis pathogenic life cycle?

Tuesday, June 21, 2011– Morning

Transcription Regulation I

CHAIR: Terry Koehler

• 2:00 – 2:15 T47, Kris Ann Baker, Dept. of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
  • Transcription anti-termination by a phosphorylated response regulator and cobalamin-dependent termination at a B12 riboswitch contribute to ethanolamine utilization in Enterococcus faecalis.

• 2:15 – 2:30 T48, Harald Putzer, Institut de Biologie Physico-Chimique, Paris, France
  • The roles of RNases Y and J in mRNA metabolism in Bacillus subtilis.

• 2:30 – 2:45 T49, Jean-Christophe Giard, Laboratoire de Microbiologie de l’Environnement, University of Caen, Caen, France
  • First experimental genome-wide identification of small non-coding RNAs in the opportunistic pathogen Enterococcus faecalis V583.

• 2:45 – 3:00 T50, Peter Lewis, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
  • Structure/Function analysis of epsilon: A new subunit of Gram positive RNA polymerases.

• 3:00 – 3:15 T51, Olivier Delumeau, INRA, Micalis Institute, Jouy-en-Josas, France
  • The dynamic protein partnership of RNA polymerase in Bacillus subtilis.

• 3:15 – 3:30 T52, Thorsten Mascher, Department of Biology, Ludwig-Maximilians-University, Munich, Germany
  • Extracytoplasmic function (ECF) sigma factors in gram-positive bacteria: Heading for the unknown.

Transcription Regulation II

CHAIR: Linc Sonenshein

• 4:00 –4:15 T53, Michael J. Federle, Dept. of Med. Chem. Pharmacognosy, The University of Illinois at Chicago, Chicago, Illinois, USA
  • Rgg transcription factors are a new class of pheromone response effectors in streptococci.

• 4:15 – 4:30 T54, Rozenn Gardan, INRA UMR1319 Micalis, Jouy-en-Josas, France
  • Rgg-associated small hydrophobic peptides: A new family of pheromones involved in quorum sensing in streptococci.

• 4:30 – 4:45 T55, Isabelle Poquet, Microbiologie de l’Alimentation au service de la Santé, UMR 1319 Micalis, Jouy-en-Josas, France
  • Lactococcus lactis ZitR is a zinc responsive repressor active in low, non-toxic zinc concentrations in vivo.

• 4:45 – 5:00 T56, Thomas Dubois, INRA, UMR1319 Micalis, Jouy-en-Josas, France
  • The NprR quorum sensor is required for the saprophytic way of life of Bacillus cereus.

• 5:00 – 5:15 T57, Boris R. Belitsky, Tufts University School of Medicine, Boston, Massachusetts, USA
  • Multiple modes of DNA binding and regulation by Bacillus subtilis CodY.

Poster Session II

• 5:30 – 7:30, Palazzo dei Congressi Sala Alba

Wednesday, June 22, 2011– Morning

Pathogenesis II

CHAIR: Agnes Fouet

• 8:30 – 8:45 T58, Hèléne Bierne, Institut Pasteur, Unité des Interactions Bactéries Cellules – Inserm, Paris, France
  • Listeria monocytogenes hijacks the chromatin-remodeling machinery to modulate type III interferon responses.

• 8:45 – 9:00 T59, Jennifer Mitchell, University College Dublin, Dublin, Ireland
  • CD36 is a receptor for the adherence of Gram positive pathogens to platelets.

• 9:00 – 9:15 T60, Marco Rinaldo Oggioni, University of Siena, Siena, Italy
  • A single bacterium at the origin of invasive pneumococcal disease: macrophage clearance defines the bottleneck during the first hours of infection.

• 9:15 – 9:30 T61, Johannes Huebner, Division of Infectious Diseases, University Hospital Freiburg, Freiburg, Germany
  • Genetic loci involved in surface carbohydrate production in Enterococci.

• 9:30 – 9:45 T62, Kimberly Kline, Dept. of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA
  • Molecular requirements for localized secretion and sortase-assembled virulence factor biogenesis in Enterococcus faecalis.

• 9:45 – 10:00 T63, Hailyn V. Nielsen, University of Washington School of Medicine, St. Louis, Missouri, USA
  • Minor tip and base pilins EbpA and EbpB contribute to E. faecalis virulence in a murine model of foreign body-associated urinary tract infection.

Antimicrobial resistance

CHAIR: Jan Maarten van Dijl

• 10:30 – 10:45 T64, Emma L. Denham, University Medical Center Groningen, Department of Medical Microbiology, Groningen, The Netherlands
  • Unraveling the bactericidal mechanism of the glycopeptides sublancin 168.

• 10:45 – 11:00 T65, Alexandra A. Roberts, School of Pharmacy, University of East Anglia, Norwich, UK
  • FosB is a bacillithiol-S-transferase that confers bacillithiol-dependent fosfomycin resistance on the Gram positive pathogen Staphylococcus aureus.

• 11:00 – 11:15 T66, Tarek Msadek, Biology of Gram-positive Pathogens, Department of Microbiology, Institut Pasteur, Paris, France
  • The ABCs of bacitracin and nisin resistance in Staphylococcus aureus.

• 11:15 – 11:30 T67, Susanne Gebhard, Department of Biology, Ludwig-Maximilians University Munich, Munich, Germany
  • Co-evolution of and signaling by ABC transporters and two-component regulatory systems as resistance modules against antimicrobial peptides in Firmicutes bacteria.

• 11:30 – 11:45 T68, Willem van Schaik, Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
  • Comprehensive identification of genes required for resistance to β-lactam antibiotics in the nosocomial pathogen Enterococcus faecium.

• 11:45 – 12:00 T69, Mary K. Phillips-Jones, Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
  • In vitro activation and inhibition of the intact quorum membrane sensor FsrC involved in virulence in Enterococcus faecalis V583

Wednesday, June 22, 2011– Afternoon

Pathogenesis III

CHAIR: Johannes Huebner

• 2:00 – 2:15 T70, Breck A. Duerkop, Department of Immunology, University of Texas, Dallas, Texas, USA
  • Bacteriophage production by Enterococcus faecalis in the mammalian intestine.

• 2:15 – 2:30 T71, Renata Matos, ITQB, Oeiras, Portugal; Institut Micalis, INRA, Jouy-en-Josas, France; Universidad Complutense de Madrid, Spain
  • Enterococcus faecalis V583 prophages are induced by low concentrations of antibiotic.

• 2:30 – 2:45 T72, Pascale S. Guiton, Dept. of Mol. Microbiol. & Microbial Pathogenesis, Washington University, St. Louis, Missouri, USA
  • Characterization of the host response to Enterococcus faecalis mediated catheter-associated urinary tract infection.

• 2:45 – 3:00 T73, Ravi P.N. Mishra, Novartis Vaccines and Diagnostics, Siena, Italy
  • Ferric Hydroxamate-binding Lipoprotein FhuD2 protects against Staphylococcus aureus infection.

Pathogenesis IV: Streptococci

CHAIR: Paul Sullam

• 3:30 – 3:45 T74, Heaven Oliver-Kozup, Dept. Microbiol., Immunol. & Cell Biol., West Virginia University, Morgantown, West Virginia, USA
  • Targeted binding of the surface adhesion Sel1 to a spliced variant of cellular fibronectin expressed in wounded tissue.

• 3:45 – 4:00 T75, Kathryn M. Gold, Dept. of Cell Biology & Molecular Genetics, University of Maryland, College Park, Maryland, USA
  • Characterization of the TrxSR Two-Component Signal Transduction System that Activates Virulence in Streptococcus pyogenes.

• 4:00 – 4:15 T76, Vijay Pancholi, Department of Pathology, The Ohio State University, Columbus, Ohio, USA
  • A novel transcriptional regulatory role of SDH/GAPDH in Streptococcus pyogenes metabolism and virulence.

• 4:15 – 4:30 T77, Sean D. Reid, Dept. Microbiol. & Immunol., Wake Forest University, Winston-Salem, North Carolina, USA
  • Insights into the regulation of Group A Streptococcal biofilm formation and dispersal and the impact on disease severity.

• 4:30 – 4:45 T78, Ravin Seepersaud, Division of Infectious Disease, University of California, San Francisco, California, USA
  • The accessory Sec protein Asp2 is required for both the optimal glycosylation and transport of GspB, the serine rich repeat glycoprotein adhesion of Streptococcus gordonii.

• 4:45 – 5:00 T79, Françoise Rul, INRA, Micalis Institute, Jouy-en-Josas, France
  • The proteomic signature of Streptococcus thermophilus in the digestive environment provides clues to lactic acid bacteria/host interactions.

• 5:00 – 5:15 T80, Philippe Glaser, Laboratoire Evolution et Génomique Bactériennes, Institut Pasteur, Paris, France
  • Emergence of a Streptococcus agalactiae lineage hypervirulent in fish by reductive evolution.

• 5:15 – 5:30 T81, Shaynoor Dramsi, Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram-Positif, Paris, France
  • Role of Streptococcus gallolyticus Pil1 pilus in endocarditis.

Thursday, June 23, 2011– Morning

Stress I

CHAIR: Erhard Bremer

• 8:30 – 8:45 T82, Terry A. Krulwich, Dept. Pharmacol. Systems Therapeutics, Mount Sinai School of Medicine, New York, New York, USA
  • In accord with the “no free lunch” principle, adaptations of alkaliphilc Bacillus that support constitutive readiness for an alkali challenge reduce fitness near neutral pH.

• 8:45 – 9:00 T83, Masahiro Ito, Graduate School of Life Science, Toyo University, Oura-gun, Gunma, Japan
  • A novel K+-stimulated flagellar motor from alkaliphilic Bacillus.

• 9:00 – 9:15 T84, Jan Maarten van Dijl, University Medical Center Groningen, Dept.of Medical Microbiology, Groningen, The Netherlands
  • Intrinsic salt-sensitivity of minimal TatAC protein translocases in Gram-positive bacteria.

• 9:15 – 9:30 T85, Veronique Broussolle, INRA, Université d’Avignon et des Pays de Vaucluse, Avignon, France
  • RNA helicases are involved in the cold adaptation of Bacillus cereus ATCC 14579.

• 9:30 – 9:45 T86, Geremy Clair, INRA, Université d’Avignon et des Pays de Vaucluse, Avignon, France
  • Bacillus cereus responds to low extracellular oxidoreduction potential by readjusting its proteome and secretome.

• 9:45 – 10:00 T87, Ulrike Mäder, Ernst-Moritz-Arndt-University, Greifswald, Germany
  • Whole-transcriptome analysis of Bacillus subtilis under various environmental conditions.

Stress II

CHAIR: Terry Krulwich

• 10:30 – 10:45 T88, Axel Hartke, Lab. Microbiologie de l’Environnement, Université de Caen, Caen, France
  • The oxidative stress response of Enterococci: Connection to virulence and antibiotic resistance.

• 10:45 – 11:00 T89, Matthieu Jules, INRA, Micalis Institute, Jouy-en-Josas, France
  • Multi-omics, multi-laboratory study unravels Bacillus dynamic responses to environmental shifts.

• 11:00 – 11:15 T90, Erhard Bremer, Microbiology, Philipps University of Marburg, Marburg, Germany
  • Regulation of the synthesis of the osmoprotectant glycine betaine in B. subtilis: Induction by choline and repression by a chemical chaperone.

• 11:15 – 11:30 T91, Mamta Rawat, California State University-Fresno, California, USA
  • Bacillithiol dependent detoxification in Staphylococcus aureus.

• 11:30 – 11:45 T92, Vanessa Khemici, Centre National de la Recherche Scientifique, Université de Toulouse, Toulouse, France
  • DNA replication stress triggers competence for genetic transformation in Streptococcus pneumoniae.