Difference between revisions of "SubtInteract"
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=== [[DNA replication]]: the [[replisome]]=== | === [[DNA replication]]: the [[replisome]]=== | ||
=== [[transcription]]: [[RNA polymerase]]=== | === [[transcription]]: [[RNA polymerase]]=== | ||
| − | ===[[translation]]: the [[ribosomal proteins|ribosome]]=== | + | === [[translation]]: the [[ribosomal proteins|ribosome]]=== |
| + | === synthesis of glutamyl-tRNA(Gln): the [[transamidosome]] ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]]=== | ||
=== [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]=== | === [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]=== | ||
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]=== | === [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]=== | ||
=== [[cell division]]: the [[divisome]]=== | === [[cell division]]: the [[divisome]]=== | ||
| − | ===DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== | + | === DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}=== |
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | === [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}=== | ||
==Important publications== | ==Important publications== | ||
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 </pubmed> | <pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660 20969605 15451506 </pubmed> | ||
Revision as of 10:03, 24 April 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Contents
- 1 Methods to detect protein-protein interactions
- 2 Visualization of protein-protein interactions in B. subtilis
- 3 Protein complexes in B. subtilis
- 3.1 DNA replication: the replisome
- 3.2 transcription: RNA polymerase
- 3.3 translation: the ribosome
- 3.4 synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
- 3.5 RNA processing and degradation: the RNA degradosome
- 3.6 general stress response: the stressosome
- 3.7 cell division: the divisome
- 3.8 DNA uptake: the pseudopilus PubMed
- 3.9 metabolism: the metabolons of glycolysis and the TCA cycle PubMed
- 4 Important publications
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- the beta version of an interactive protein-protein interaction map (just replace "PtsH" in the browser line by the protein of your interest)
- the beta version of SubtInteract
Protein complexes in B. subtilis
DNA replication: the replisome
transcription: RNA polymerase
translation: the ribosome
synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
RNA processing and degradation: the RNA degradosome
general stress response: the stressosome
cell division: the divisome
DNA uptake: the pseudopilus PubMed
metabolism: the metabolons of glycolysis and the TCA cycle PubMed
Important publications
Agnès Vendeville, Damien Larivière, Eric Fourmentin
An inventory of the bacterial macromolecular components and their spatial organization.
FEMS Microbiol Rev: 2011, 35(2);395-414
[PubMed:20969605]
[WorldCat.org]
[DOI]
(I p)
Mike P Williamson, Michael J Sutcliffe
Protein-protein interactions.
Biochem Soc Trans: 2010, 38(4);875-8
[PubMed:20658969]
[WorldCat.org]
[DOI]
(I p)
Patrick Amar, Guillaume Legent, Michel Thellier, Camille Ripoll, Gilles Bernot, Thomas Nystrom, Milton H Saier, Vic Norris
A stochastic automaton shows how enzyme assemblies may contribute to metabolic efficiency.
BMC Syst Biol: 2008, 2;27
[PubMed:18366733]
[WorldCat.org]
[DOI]
(I e)
Adam Brymora, Valentina A Valova, Phillip J Robinson
Protein-protein interactions identified by pull-down experiments and mass spectrometry.
Curr Protoc Cell Biol: 2004, Chapter 17;Unit 17.5
[PubMed:18228443]
[WorldCat.org]
[DOI]
(I p)
Hongtao Guan, Endre Kiss-Toth
Advanced technologies for studies on protein interactomes.
Adv Biochem Eng Biotechnol: 2008, 110;1-24
[PubMed:18219467]
[WorldCat.org]
[DOI]
(I p)
Vincent Collura, Guillaume Boissy
From protein-protein complexes to interactomics.
Subcell Biochem: 2007, 43;135-83
[PubMed:17953394]
[WorldCat.org]
[DOI]
(P p)
Philippe Noirot, Marie-Françoise Noirot-Gros
Protein interaction networks in bacteria.
Curr Opin Microbiol: 2004, 7(5);505-12
[PubMed:15451506]
[WorldCat.org]
[DOI]
(P p)
Barry Causier
Studying the interactome with the yeast two-hybrid system and mass spectrometry.
Mass Spectrom Rev: 2004, 23(5);350-67
[PubMed:15264234]
[WorldCat.org]
[DOI]
(P p)
Andrzej Dziembowski, Bertrand Séraphin
Recent developments in the analysis of protein complexes.
FEBS Lett: 2004, 556(1-3);1-6
[PubMed:14706816]
[WorldCat.org]
[DOI]
(P p)
Alfonso Valencia, Florencio Pazos
Computational methods for the prediction of protein interactions.
Curr Opin Struct Biol: 2002, 12(3);368-73
[PubMed:12127457]
[WorldCat.org]
[DOI]
(P p)
Peter Uetz
Two-hybrid arrays.
Curr Opin Chem Biol: 2002, 6(1);57-62
[PubMed:11827824]
[WorldCat.org]
[DOI]
(P p)
P Legrain, J Wojcik, J M Gauthier
Protein--protein interaction maps: a lead towards cellular functions.
Trends Genet: 2001, 17(6);346-52
[PubMed:11377797]
[WorldCat.org]
[DOI]
(P p)
C L Tucker, J F Gera, P Uetz
Towards an understanding of complex protein networks.
Trends Cell Biol: 2001, 11(3);102-6
[PubMed:11306254]
[WorldCat.org]
[DOI]
(P p)
P Uetz, R E Hughes
Systematic and large-scale two-hybrid screens.
Curr Opin Microbiol: 2000, 3(3);303-8
[PubMed:10851163]
[WorldCat.org]
[DOI]
(P p)
Z Chen, M Han
Building a protein interaction map: research in the post-genome era.
Bioessays: 2000, 22(6);503-6
[PubMed:10842303]
[WorldCat.org]
[DOI]
(P p)
P A Srere
Macromolecular interactions: tracing the roots.
Trends Biochem Sci: 2000, 25(3);150-3
[PubMed:10694888]
[WorldCat.org]
[DOI]
(P p)
P A Srere, J Ovadi
Enzyme-enzyme interactions and their metabolic role.
FEBS Lett: 1990, 268(2);360-4
[PubMed:2200717]
[WorldCat.org]
[DOI]
(P p)
P A Srere
Complexes of sequential metabolic enzymes.
Annu Rev Biochem: 1987, 56;89-124
[PubMed:2441660]
[WorldCat.org]
[DOI]
(P p)
