Difference between revisions of "YpbH"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' GP812 (spc), GP814 (spc) both available in the [[Stülke]] lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 15:47, 7 February 2011
- Description: putative adaptor protein
| Gene name | ypbH |
| Synonyms | |
| Essential | no |
| Product | putative adaptor protein |
| Function | unknown |
| MW, pI | 22 kDa, 4.095 |
| Gene length, protein length | 582 bp, 194 aa |
| Immediate neighbours | gudB, ypbG |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22970
Phenotypes of a mutant
deletion reduces sporulation to 20%, overexpression abolishes sporulation PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): MecA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: P50734
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- low expression, enhanced in stat phase in LB or competence but not in sporulation medium PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP812 (spc), GP814 (spc) both available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Feng Wang, Ziqing Mei, Yutao Qi, Chuangye Yan, Siheng Xiang, Zhiyuan Zhou, Qi Hu, Jiawei Wang, Yigong Shi
Crystal structure of the MecA degradation tag.
J Biol Chem: 2009, 284(49);34376-81
[PubMed:19801546]
[WorldCat.org]
[DOI]
(I p)
Marjan Persuh, Ines Mandic-Mulec, David Dubnau
A MecA paralog, YpbH, binds ClpC, affecting both competence and sporulation.
J Bacteriol: 2002, 184(8);2310-3
[PubMed:11914365]
[WorldCat.org]
[DOI]
(P p)
