CitT

• Description: two-component response regulator, regulation of citrate uptake
  
  

Gene name	citT
Synonyms	yflQ
Essential	no
Product	two-component response regulator
Function	regulation of citrate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	25 kDa, 6.362
Gene length, protein length	678 bp, 226 aa
Immediate neighbours	citS, yflP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

The CitT regulon:

• citM-yflN

The gene

Basic information

• Locus tag: BSU07590

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the promoter of the citM-yflN operon and activation of transcription in the presence of citrate PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by CitS on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions: CitS-CitT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: citS-citT-yflP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: