Difference between revisions of "Nfo"
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[DNA repair/ recombination]]}}, | ||
| + | {{SubtiWiki category|[[sporulation proteins]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[SigG regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 21:27, 8 December 2010
- Description: type IV apurinic/apyrimidinic endonuclease
| Gene name | nfo |
| Synonyms | yqfS |
| Essential | no |
| Product | type IV apurinic/apyrimidinic endonuclease |
| Function | repair of oxidative DNA damage in spores |
| MW, pI | 32 kDa, 5.371 |
| Gene length, protein length | 891 bp, 297 aa |
| Immediate neighbours | yqfT, cshB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25130
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to Swiss-Prot)
- Protein family: AP endonuclease 2 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P54476
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
The gene yqfT is located between nfo and yqfU, but is transcribed in the opposite direction.
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Marcelo Barraza-Salas, Juan R Ibarra-Rodríguez, Silvia J Mellado, José M Salas-Pacheco, Peter Setlow, Mario Pedraza-Reyes
Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores.
FEMS Microbiol Lett: 2010, 302(2);159-65
[PubMed:19930460]
[WorldCat.org]
[DOI]
(I p)
Juan R Ibarra, Alma D Orozco, Juan A Rojas, Karina López, Peter Setlow, Ronald E Yasbin, Mario Pedraza-Reyes
Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores.
J Bacteriol: 2008, 190(6);2031-8
[PubMed:18203828]
[WorldCat.org]
[DOI]
(I p)
José M Salas-Pacheco, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage.
J Bacteriol: 2005, 187(21);7374-81
[PubMed:16237020]
[WorldCat.org]
[DOI]
(P p)
José M Salas-Pacheco, Norma Urtiz-Estrada, Guadalupe Martínez-Cadena, Ronald E Yasbin, Mario Pedraza-Reyes
YqfS from Bacillus subtilis is a spore protein and a new functional member of the type IV apurinic/apyrimidinic-endonuclease family.
J Bacteriol: 2003, 185(18);5380-90
[PubMed:12949090]
[WorldCat.org]
[DOI]
(P p)
Norma Urtiz-Estrada, José M Salas-Pacheco, Ronald E Yasbin, Mario Pedraza-Reyes
Forespore-specific expression of Bacillus subtilis yqfS, which encodes type IV apurinic/apyrimidinic endonuclease, a component of the base excision repair pathway.
J Bacteriol: 2003, 185(1);340-8
[PubMed:12486072]
[WorldCat.org]
[DOI]
(P p)
