MutM

• Description: formamidopyrimidine-DNA glycosidase
  
  

Gene name	mutM
Synonyms	ytaE
Essential	no
Product	formamidopyrimidine-DNA glycosidase
Function	DNA repair
MW, pI	31 kDa, 8.889
Gene length, protein length	834 bp, 278 aa
Immediate neighbours	ytaF, polA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29080

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

DNA repair/ recombination

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (according to Swiss-Prot)

• Protein family: FPG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2F5S (complex with oxoG:C, Geobacillus stearothermophilus)

• UniProt: O34403

• KEGG entry: [2]

• E.C. number: 3.2.2.23

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yan Qi, Marie C Spong, Kwangho Nam, Martin Karplus, Gregory L Verdine
Entrapment and structure of an extrahelical guanine attempting to enter the active site of a bacterial DNA glycosylase, MutM.
J Biol Chem: 2010, 285(2);1468-78
[PubMed:19889642] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)