IspE

• Description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
  
  

Gene name	ispE
Synonyms	yabH
Essential	yes PubMed
Product	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Function	isoprenoid biosynthesis
MW, pI	31 kDa, 6.349
Gene length, protein length	867 bp, 289 aa
Immediate neighbours	sspF, purR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00460

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

biosynthesis of lipids, essential genes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)

• Protein family: IspE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37550

• KEGG entry: [2]

• E.C. number: 2.7.1.148

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)