Difference between revisions of "LcfA"
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| − | * '''Description:''' long chain acyl-CoA synthetase <br/><br/> | + | * '''Description:''' long chain acyl-CoA synthetase, involved in surfactin production <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot) | + | * '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis {{PubMed|20797616}} |
* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot) | * '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot) | ||
| − | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' [[LcfB]], [[YhfT]], [[YngI]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}} |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 125: | Line 125: | ||
=References= | =References= | ||
| − | <pubmed>17189250,17919287</pubmed> | + | <pubmed>17189250,17919287 20797616</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:21, 1 September 2010
- Description: long chain acyl-CoA synthetase, involved in surfactin production
| Gene name | lcfA |
| Synonyms | |
| Essential | no |
| Product | long chain acyl-CoA synthetase |
| Function | fatty acid degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation | |
| MW, pI | 62 kDa, 6.119 |
| Gene length, protein length | 1680 bp, 560 aa |
| Immediate neighbours | fadR, yshE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28560
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
- Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P94547
- KEGG entry: [3]
- E.C. number: 6.2.1.3
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: available in Mohamed Marahiel's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
