Difference between revisions of "PrkD"
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02030] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02030] | ||
| − | * '''E.C. number:''' | + | * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.11.1 2.7.11.1] |
=== Additional information=== | === Additional information=== | ||
Revision as of 10:07, 4 August 2010
- Description: protein kinase D
| Gene name | prkD |
| Synonyms | ybdM |
| Essential | no |
| Product | protein kinase D |
| Function | unknown |
| MW, pI | 29 kDa, 6.359 |
| Gene length, protein length | 768 bp, 256 aa |
| Immediate neighbours | ybdL, ybdN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU02030
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: autophosphorylated on Thr-174 (in vitro) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O31435
- KEGG entry: [2]
- E.C. number: 2.7.11.1
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP578 (aphA3), available in the Stülke lab
- Expression vector:
- pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nico Pietack, Dörte Becher, Sebastian R Schmidl, Milton H Saier, Michael Hecker, Fabian M Commichau, Jörg Stülke
In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism.
J Mol Microbiol Biotechnol: 2010, 18(3);129-40
[PubMed:20389117]
[WorldCat.org]
[DOI]
(I p)
Edwige Madec, Agnieszka Laszkiewicz, Adam Iwanicki, Michal Obuchowski, Simone Séror
Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes.
Mol Microbiol: 2002, 46(2);571-86
[PubMed:12406230]
[WorldCat.org]
[DOI]
(P p)
