Difference between revisions of "FbaA"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
+ | ** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1423, available in [[Stülke]] lab | ||
** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP88, available in [[Stülke]] lab | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP88, available in [[Stülke]] lab | ||
** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP395, available in [[Stülke]] lab | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP395, available in [[Stülke]] lab |
Revision as of 21:44, 18 March 2010
- Description: fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme
Gene name | fbaA |
Synonyms | fba, fba1, tsr |
Essential | yes |
Product | fructose-1,6-bisphosphate aldolase |
Function | enzyme in glycolysis/ gluconeogenesis |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
MW, pI | 30,2 kDa, 5.03 |
Gene length, protein length | 855 bp, 285 amino acids |
Immediate neighbours | ywjH, spo0F |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU37120
Phenotypes of a mutant
- Essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)
- Protein family: class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
- Paralogous protein(s): FbaB
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
- Modification: phosphorylation on Thr-212 and Thr-234 PubMed
- Cofactor(s): Zn2+ (Metalloenzyme)
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: P13243
- KEGG entry: [3]
- E.C. number: 4.1.2.13
Additional information
Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
Expression and regulation
- Sigma factor:
- Regulation: constitutively expressed PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- for expression in B. subtilis, in pBQ200: pGP1423, available in Stülke lab
- for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP88, available in Stülke lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP395, available in Stülke lab
- lacZ fusion: pGP601 (in pAC6)
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References