YabT

• Description: similar to Ser/Thr kinase
  
  

Gene name	yabT
Synonyms
Essential	no
Product	unknown
Function	unknown
MW, pI	37 kDa, 9.943
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	yabS, tilS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00660

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37562

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor: SigF PubMed

• Regulation: expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP577 (erm), available in Stülke lab

• Expression vector:
  • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Stülke lab
  • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Stülke lab
  • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Stülke lab

• lacZ fusion:
• translational lacZ fusion (in pAC7): pGP831, available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)