DnaE

• Description: DNA polymerase III (alpha subunit)
  
  

Gene name	dnaE
Synonyms
Essential	yes PubMed
Product	DNA polymerase III (alpha subunit)
Function	DNA replication
MW, pI	125 kDa, 6.02
Gene length, protein length	3345 bp, 1115 aa
Immediate neighbours	ytsJ, ytrH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29230

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: DnaE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34623

• KEGG entry: [3]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon: dnaE (according to DBTBS)

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: