CsrA

• Description: similar to E. coli carbon storage regulator
  
  

Gene name	csrA
Synonyms	yviG
Essential	no
Product	unknown
Function	unknown
MW, pI	8 kDa, 6.09
Gene length, protein length	222 bp, 74 aa
Immediate neighbours	hag, fliW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: csrA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CsrA-RsmY (hypothetical)

• Localization:

Database entries

• Structure: 1T3O

• UniProt: P33911

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP469 (spc), available in Stülke lab

• Expression vector:
  • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab

• lacZ fusion: pGP461 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Prajna R Kulkarni, Xiaohui Cui, Joshua W Williams, Ann M Stevens, Rahul V Kulkarni
Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri.
Nucleic Acids Res: 2006, 34(11);3361-9
[PubMed:16822857] [WorldCat.org] [DOI] (I e)