Difference between revisions of "PAC7"

Revision as of 18:36, 7 September 2009

pAC7: click to enlarge

pAC7 cloning region: click to enlarge

vector for the construction of translational lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: kanamycin resistance

similar vectors: pAC5, pAC6

sequencing primer:

@@ Line 20: / Line 20: @@
 *'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
+<pubmed> 1715859 </pubmed>
-Weinrauch et al. (1991) J Bacteriol.  173:5685-5693. [http://www.ncbi.nlm.nih.gov/sites/entrez/1715859  PubMed]