PurR

• Description: transcription repressor of the pur operon
  
  

Gene name	purR
Synonyms	yabI
Essential	no
Product	transcription repressor of the pur operon
Function	regulation of purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	31 kDa, 5.771
Gene length, protein length	855 bp, 285 aa
Immediate neighbours	ispE, yabJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Biological activity: transcriptional repressor of purine biosynthetic operons

• Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed

• Interactions: PurR-Prs

• Localization:

Database entries

• Structure: 1P4A, 1P4A (complex with PRPP) NCBI PubMed

• Swiss prot entry: P37551

• KEGG entry: [3]

• E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

• Operon: purR-yabJ PubMed

• Sigma factor:

• Regulation: negative autoregulation, induction in the presence of PRPP PubMed
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism: repression by PurR PubMed
  • PurR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

Aloke Kumar Bera, Jianghai Zhu, Howard Zalkin, Janet L Smith
Functional dissection of the Bacillus subtilis pur operator site.
J Bacteriol: 2003, 185(14);4099-109
[PubMed:12837784] [WorldCat.org] [DOI] (P p)

Sangita C Sinha, Joseph Krahn, Byung Sik Shin, Diana R Tomchick, Howard Zalkin, Janet L Smith
The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.
J Bacteriol: 2003, 185(14);4087-98
[PubMed:12837783] [WorldCat.org] [DOI] (P p)

M Weng, H Zalkin
Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.
Curr Microbiol: 2000, 41(1);56-9
[PubMed:10919400] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed