OpuE

• Description: proline transporter
  
  

Gene name	opuE
Synonyms	yerK
Essential	no
Product	proline transporter
Function	compatible solute transport
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	53 kDa, 9.815
Gene length, protein length	1476 bp, 492 aa
Immediate neighbours	sapB, gatC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06660

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s): YcgO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O06493

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

1. von Blohn C, Kempf B, Kappes RM, Bremer E. (1997) Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B. Mol Microbiol. Jul;25(1): 175-87. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed