Difference between revisions of "LevR"

From SubtiWiki
Jump to: navigation, search
Line 13: Line 13:
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of levan and fructose utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of levan and fructose utilization
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 105 kDa, 5.943   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 105 kDa, 5.943   

Revision as of 11:53, 11 June 2009

Gene name levR
Synonyms
Essential no
Product transcriptional activator, PRD-type (for SigL-dependent promoter)
Function regulation of levan and fructose utilization
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 105 kDa, 5.943
Gene length, protein length 2814 bp, 938 aa
Immediate neighbours levD, aapA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LevR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU27080

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binding to the upstream activating sequence in front of the levD gene results in activation of transcription of the levD-levE-levF-levG-sacC operon, bining occurs in the presence of fructose
  • Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot), Attention: this is wrong: LicR is a transcription activator, the similarity to the BglG family is limited to the PTS-regulation domains
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Débarbouillé M, Martin-Verstraete I, Klier A, Rapoport G (1991) The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both (Sigma)54 - and phosphotransferase system-dependent regulators. Proc Natl Acad Sci USA 88:2212-2216. PubMed
  2. Stülke, J., Martin-Verstraete, I., Charrier, V., Klier, A., Deutscher, J. & Rapoport, G. (1995) The HPr protein of the phosphotransferase system links induction and catabolite repression of the Bacillus subtilis levanase operon. J. Bacteriol. 177: 6928-6936. PubMed
  3. Martin-Verstraete, I., Stülke, J., Klier, A. & Rapoport, G. (1995) Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon. J. Bacteriol. 177: 6919-6927. PubMed
  4. Martin-Verstraete, I., Charrier, V., Stülke, J., Galinier, A., Erni, B., Rapoport, G., & Deutscher, J. (1998) Antagonistic effects of dual PTS catalyzed phosphorylation on the Bacillus subtilis transcriptional activator LevR. Mol. Microbiol. 28: 293-303. PubMed
  5. Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G (1994) Interactions of wild type and truncated LevR of Bacillus subtilis with the upstream activating sequence of the levanase operon. J Mol Biol 241:178-192. PubMed
  6. Charrier V, Deutscher J, Martin-Verstraete I (1997b) Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon. Biochemistry 36:1163-1172. PubMed
  7. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed