AraR

• Description: repressor of the arabinose utilization genes
  
  

Gene name	araR
Synonyms	araC, yvbS
Essential	no
Product	transcriptional repressor (LacI family))
Function	regulation of arabinose utilization (araABDLMNPQ-abfA) and of the araE/araR genes
MW, pI	43 kDa, 6.341
Gene length, protein length	1152 bp, 384 aa
Immediate neighbours	araE, yvbT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Genes/ operons controlled by AraR

• Activated by AraR:
• Repressed by AraR: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P96711

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed in the absence of arabinose (AraR) PubMed

• Regulatory mechanism:
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, L J Mota
Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene.
J Bacteriol: 1997, 179(5);1598-608
[PubMed:9045819] [WorldCat.org] [DOI] (P p)

1. Mota LJ, Tavares P, Sá-Nogueira I. (1999) Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis. Mol Microbiol. Aug;33(3): 476-89. PubMed
2. Sá-Nogueira I, Mota LJ. (1997) Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. J Bacteriol. Mar;179(5): 1598-608. PubMed