Difference between revisions of "TsaE"
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=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU05910 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 82: | Line 82: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05515 O05515] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05515 O05515] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU05910] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Revision as of 10:21, 3 June 2009
- Description: P-loop ATPase
| Gene name | ydiB |
| Synonyms | |
| Essential | no |
| Product | P-loop ATPase |
| Function | unknown |
| MW, pI | 17 kDa, 4.597 |
| Gene length, protein length | 474 bp, 158 aa |
| Immediate neighbours | thiL, ydiC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU05910
Phenotypes of a mutant
reduced growth rate PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: the corresponding E. coli protein is involved in the control of the activity of the Gcp homolog PubMed
- Protein family: UPF0079 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: K(M) for ATP: 60 myM, V(max) 10 nmol/min PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: dimerizes and oligomerizes PubMed, the corresponding E. coli protein interacts with the protein corresponding to YdiC PubMed
- Localization: cytoplasm with the excepton of the nucleoid region PubMed
Database entries
- Structure:
- Swiss prot entry: O05515
- KEGG entry: [2]
- E.C. number:
Additional information
ATPase activity is required for in vivo function of the protein PubMed
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP833 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Johanna C Karst, Anne-Emmanuelle Foucher, Tracey L Campbell, Anne-Marie Di Guilmi, David Stroebel, Chand S Mangat, Eric D Brown, Jean-Michel Jault
The ATPase activity of an 'essential' Bacillus subtilis enzyme, YdiB, is required for its cellular function and is modulated by oligomerization.
Microbiology (Reading): 2009, 155(Pt 3);944-956
[PubMed:19246765]
[WorldCat.org]
[DOI]
(P p)
Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971]
[WorldCat.org]
[DOI]
(P p)
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
