Difference between revisions of "Sandbox"

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* '''Description:''' O6-methylguanine-DNA methyltransferase <br/><br/>
+
* '''Description:''' adenine deaminase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''adaB''
+
|''adeC''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ade, yzaD ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || O6-methylguanine-DNA methyltransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || adenine deaminase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || adaptive response to alkylative DNA damage
+
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of adenine as nitrogen source, purine salvage and interconversion
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 6.152  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 4.724  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 537 bp, 179 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1731 bp, 577 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[adaA]]'', ''[[ndhF]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ktrC]]'', ''[[rnjA]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11958&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13325&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:adaB_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:adeC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 37:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU01820
+
* '''Locus tag:''' BSU14520
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 43:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/adaAB.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10167]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11044]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' Adenine + H<sub>2</sub>O = hypoxanthine + NH<sub>3</sub> (according to Swiss-Prot)  
  
* '''Protein family:''' MGMT family (according to Swiss-Prot)
+
* '''Protein family:''' adenine deaminase family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 78: Line 80:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P19220 P19220]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39761 P39761]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU01820]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14520]
  
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.1.63 2.1.1.63]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.5.4.2 3.5.4.2]
  
 
=== Additional information===
 
=== Additional information===

Revision as of 22:27, 31 May 2009

  • Description: adenine deaminase

Gene name adeC
Synonyms ade, yzaD
Essential no
Product adenine deaminase
Function utilization of adenine as nitrogen source, purine salvage and interconversion
MW, pI 62 kDa, 4.724
Gene length, protein length 1731 bp, 577 aa
Immediate neighbours ktrC, rnjA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AdeC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU14520

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Adenine + H2O = hypoxanthine + NH3 (according to Swiss-Prot)
  • Protein family: adenine deaminase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed