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| − | * '''Description:''' nitrogen-regulated PII-like protein <br/><br/>
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''nrgB''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || nitrogen-regulated PII-like protein
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of ammonium uptake
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 8.825
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 348 bp, 116 aa
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrgA]]'', ''[[bcrC]]''
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| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nrgB_nucleotide.txt Gene sequence (+200bp) ]'''
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| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nrgB_protein.txt Protein sequence]'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:nrgB_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |}
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| − | __TOC__
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| − | <br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Coordinates:'''
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| − | ===Phenotypes of a mutant ===
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nrgAB.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10870]
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| − | === Additional information===
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:'''
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| − | * '''Protein family:''' P(II) protein family (according to Swiss-Prot)
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| − | * '''Paralogous protein(s):'''
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | * '''Interactions:''' [[NrgA]]-[[NrgB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/14600241 PubMed], [[TnrA]]-[[NrgB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17001076 PubMed]
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| − | * '''Localization:''' cell membrane (according to Swiss-Prot), membrane
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| − | === Database entries ===
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| − | * '''Structure:'''
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| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q07428 Q07428]
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU36520 BSU36520]
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| − | * '''E.C. number:'''
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| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[nrgA]]-[[nrgB]]''
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| − | * '''[[Sigma factor]]:''' [[SigA]]
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| − | * '''Regulation:''' induced by ammonium limitation
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| − | * '''Regulatory mechanism:''' transcription activation by [[TnrA]]
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| − | * '''Additional information:'''
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| − | =Biological materials =
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| − | * '''Mutant:'''
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| − | * '''Expression vector:'''
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:''' available in [[Jörg Stülke]] lab
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| − | =Labs working on this gene/protein=
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| − | [[Susan Fisher]], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | # Atkinson MR, Fisher SH (1991) Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J Bacteriol 173:23-27. [http://www.ncbi.nlm.nih.gov/sites/entrez/1670935 PubMed]
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| − | # Wray, L. V., Atkinson, M. R. & Fisher, S. H. (1994). The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein. J Bacteriol 176, 108-114. [http://www.ncbi.nlm.nih.gov/sites/entrez/8282685 PubMed]
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| − | # Detsch, C. & Stülke, J. (2003) Ammonium utilization in ''Bacillus subtilis'': transport and regulatory functions of NrgA and NrgB. Microbiology 149: 3289-3297. [http://www.ncbi.nlm.nih.gov/sites/entrez/14600241 PubMed]
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| − | # Heinrich, A., Woyda, K., Brauburger, K., Meiss, G., Detsch, C., Stülke, J. & Forchhammer, K. (2006) Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in ''Bacillus subtilis''. J. Biol. Chem. 281: 34909-34917. [http://www.ncbi.nlm.nih.gov/sites/entrez/17001076 PubMed]
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| − | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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