Difference between revisions of "Sandbox"

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* '''Description:''' phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate) <br/><br/>
+
* '''Description:''' UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''mraY''
+
|''murD''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || peptidoglycan precursor biosynthesis  
+
|style="background:#ABCDEF;" align="center"|'''Function''' || peptidoglycan precursor biosynthesis
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 8.966  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 5.566  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 972 bp, 324 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1353 bp, 451 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[murE]]'', ''[[murD]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mraY]]'', ''[[spoVE]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13392&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13393&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:mraY_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:murD_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10224]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10225]
  
 
=== Additional information===
 
=== Additional information===
Line 56: Line 58:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' MraY subfamily (according to Swiss-Prot)
+
* '''Protein family:''' murCDEF family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:''' cytoplasm (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q03521 Q03521]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q03522 Q03522]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15190]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15200]
  
−
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.13 2.7.8.13]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.2.9 6.3.2.9]
  
 
=== Additional information===
 
=== Additional information===
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=References=
 
=References=
  
−
# Al-Dabbagh B, Henry X, El Ghachi M, Auger G, Blanot D, Parquet C, Mengin-Lecreulx D, Bouhss A. (2008) Active site mapping of MraY, a member of the polyprenyl-phosphate N-acetylhexosamine 1-phosphate transferase superfamily, catalyzing the first membrane step of peptidoglycan biosynthesis. ''Biochemistry.'' '''Aug 26;47(34):''' 8919-28.  [http://www.ncbi.nlm.nih.gov/sites/entrez/18672909 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 22:57, 11 May 2009

  • Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene name murD
Synonyms
Essential yes PubMed
Product UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Function peptidoglycan precursor biosynthesis
MW, pI 49 kDa, 5.566
Gene length, protein length 1353 bp, 451 aa
Immediate neighbours mraY, spoVE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MurD context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: murCDEF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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